Related Experiment Video
Updated: Jun 26, 2026
08:01
Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection
Published on: December 15, 2015
HPLC in natural product analysis: the detection issue
1Laboratory of Pharmacognosy and Phytochemistry, School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Geneva, Switzerland. jean-luc.wolfender@unige.ch
Planta Medica
|January 16, 2009
Summary
High-performance liquid chromatography (HPLC) is a versatile method for analyzing natural products (NPs). This review covers various HPLC detectors for NP separation, detection, and quantification in complex samples.
Area of Science:
- Analytical Chemistry
- Natural Product Chemistry
Background:
- High-performance liquid chromatography (HPLC) is a widely adopted technique for natural product (NP) analysis.
- HPLC offers versatility in separating diverse NPs from complex matrices like crude extracts.
- Minimal sample preparation is often required for NP analysis using HPLC.
Purpose of the Study:
- To review qualitative and quantitative applications of HPLC for natural product (NP) analysis.
- To provide an overview of various detection methods used in conjunction with HPLC for NPs.
- To discuss the potential, limitations, and emerging trends in HPLC hyphenation for NP research.
Main Methods:
- Review of scientific literature on HPLC applications for natural product (NP) separation and detection.
- Compilation of data on various HPLC detectors including UV, DAD, FD, ECD, RID, FID, CL, ESLD, CAD, MS, MS-MS, and NMR.
- Discussion of qualitative and quantitative analytical strategies.
Main Results:
- HPLC is effective for selective detection, quantification, and profiling of NPs.
- The choice of detector significantly impacts NP analysis due to their diverse chemical properties.
- A wide array of detectors can be coupled with HPLC for comprehensive NP characterization.
Conclusions:
- HPLC coupled with diverse detectors provides powerful tools for natural product (NP) research.
- Understanding detector capabilities and limitations is crucial for effective NP analysis.
- Advancements in HPLC hyphenation continue to enhance the study of natural products.
More Related Videos
Related Concept Videos
01:15High-Performance Liquid Chromatography: Types of Detectors
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...
01:11High-Performance Liquid Chromatography: Introduction
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
In HPLC, two phases play a critical role in the separation process:
00:57High-Performance Liquid Chromatography: Instrumentation
High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
01:05High-Performance Liquid Chromatography: Elution Process
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
01:13Gas Chromatography: Overview of Detectors
Detectors in gas chromatography (GC) help identify and quantify the components of a mixture by translating chemical properties into measurable signals, which are displayed on a chromatogram. Detectors can be categorized into two main types: destructive and non-destructive.
A non-destructive detector allows a sample to be analyzed without altering or consuming it, meaning the sample can be collected after detection for further analysis. Examples include thermal conductivity detectors and...
A non-destructive detector allows a sample to be analyzed without altering or consuming it, meaning the sample can be collected after detection for further analysis. Examples include thermal conductivity detectors and...