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Visualizing Hyporheic Flow Through Bedforms Using Dye Experiments and Simulation

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Published on: November 18, 2015

Extra-column dead volume in simulated moving bed separations: theory and experiments.

Shigeharu Katsuo¹, Christian Langel, Patrick Schanen

¹Institute of Process Engineering, ETH Zurich, Sonneggstrasse 3, CH-8092 Zurich, Switzerland.

Journal of Chromatography. A

|January 17, 2009

Summary

This summary is machine-generated.

Extra-column dead volume in small-scale Simulated Moving Bed (SMB) chromatography significantly impacts separation. This study provides guidelines to account for dead volume, optimizing operating parameters for better results.

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Curtain Flow Column: Optimization of Efficiency and Sensitivity

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Published on: June 12, 2016

Area of Science:

Chromatographic separation science
Chemical engineering

Background:

In small-scale Simulated Moving Bed (SMB) chromatography, the volume of connecting tubing and valves can be substantial relative to column volume.
This extra-column dead volume can compromise separation efficiency and accuracy.

Purpose of the Study:

To investigate the impact of extra-column dead volume on separation performance in HPLC-SMB units.
To develop practical guidelines and simple rules for accounting for dead volume in operating parameter calculations.

Main Methods:

Experimental separation of a racemic mixture using a laboratory HPLC-SMB unit.
Numerical modeling explicitly incorporating the geometric configuration to simulate dead volume effects.

Main Results:

Demonstrated the significant influence of extra-column dead volume on separation outcomes.
Provided a framework, using triangle theory, to determine optimal operating conditions considering dead volume.

Conclusions:

Accounting for extra-column dead volume is crucial for achieving satisfactory separation in small-scale HPLC-SMB systems.
The developed guidelines facilitate the optimization of operating parameters for enhanced chromatographic performance.