Related Experiment Video
Updated: Jun 26, 2026
12:21
MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues
Published on: November 30, 2013
Using in situ hybridisation to localize renal gene expression in tissue sections
Ian A Darby1, Alexis Desmoulière, Tim D Hewitson
1Department of Nephrology, School of Medical Sciences, RMIT University, Bundoora, VIC, Australia.
Methods in Molecular Biology (Clifton, N.J.)
|January 17, 2009
Summary
In situ hybridization precisely maps nucleic acid distribution, crucial for understanding gene expression patterns in the kidney. This technique remains vital despite advances in other molecular methods.
Area of Science:
- Molecular Biology
- Genetics
- Anatomy
Background:
- In situ hybridization is a fundamental technique for localizing nucleic acids within biological samples.
- Despite advancements like gene microarrays, the need for precise spatial gene expression analysis persists.
- In situ hybridization provides unparalleled precision in identifying the topology of gene expression.
Purpose of the Study:
- To outline methods for examining the spatial distribution of gene expression in the kidney.
- To detail the use of complementary RNA (cRNA) probes for gene expression analysis.
- To cover both radioactive and non-radioactive labeling techniques for in situ hybridization.
Main Methods:
- Utilizing complementary RNA (cRNA) probes for in situ hybridization.
- Employing both radioactive and non-radioactive labeling strategies.
- Focusing on the spatial localization of nucleic acids within kidney tissue.
Main Results:
- Demonstration of techniques for precise spatial mapping of gene expression in the kidney.
- Successful application of cRNA probes for identifying gene distribution.
- Validation of both radioactive and non-radioactive labeling methods.
Conclusions:
- In situ hybridization is an indispensable tool for detailed gene expression analysis in the kidney.
- The spatial resolution of in situ hybridization complements other molecular techniques.
- This chapter provides a practical guide to applying in situ hybridization in renal research.
Related Concept Videos
02:31In-situ Hybridization
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
02:07FISH - Fluorescent In-situ Hybridization
Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...