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Optical Clearing and Imaging of Immunolabeled Kidney Tissue
Published on: July 22, 2019
Immuno and lectin histochemistry for renal light microscopy
Tim D Hewitson1, Lauren Grimwood
1Department of Nephrology, The Royal Melbourne Hospital and Department of Medicine, University of Melbourne, Melbourne, Victoria, Australia.
Methods in Molecular Biology (Clifton, N.J.)
|January 17, 2009
Summary
Histochemistry techniques, including immunohistochemistry, are vital for analyzing cell and tissue biology, especially for complex organs like the kidney. This chapter details standard methods for localizing antigens and carbohydrates in fixed paraffin-embedded tissues.
Area of Science:
- Cell biology
- Tissue biology
- Histochemistry
Background:
- Histochemistry is fundamental for analyzing cell and tissue biology.
- Advancements in staining techniques enhance the resolution for studying complex organ structures, such as the kidney.
Purpose of the Study:
- To detail standard histochemical techniques for antigen and carbohydrate localization.
- To focus on immunohistochemistry with fixed paraffin-embedded tissue sections for research applications.
Main Methods:
- Immunohistochemistry for antigen localization.
- Lectin binding for carbohydrate localization.
- Utilizing fixed paraffin-embedded tissue sections.
Main Results:
- Standardized histochemical protocols enable precise localization of cellular components.
- The described methods are applicable to complex organ structures like the kidney.
- Immunohistochemistry provides high-resolution analysis of fixed paraffin-embedded tissues.
Conclusions:
- Standard histochemical techniques, particularly immunohistochemistry, are essential tools in modern cell and tissue biology research.
- These methods facilitate detailed analysis of organ structures and are adaptable for various research laboratory settings.
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