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Updated: Jun 26, 2026

Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants
Published on: June 13, 2014
[Cloning and functional analysis of two regulators involved in regulation of cellulase formation in Trichoderma
Min Ling1, Yongling Qin, Nan Li
1Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization, College of Life Science and Technology, Guangxi University, Nanning 530004, China.
Abstract:
ACEI and Xyr1 are two regulators that potentially involve in regulation of cellulases and xylanases formation in Trichoderma reesei, they compete for a binding site in the xyn1 (Xylanase1-encoding) gene promoter. To further investigate the mechanism for the transcriptional regulation of cellulases, DNA-binding domains of both ACEI and Xyr1 in T. Koningii were expressed from E. coli. They both showed bindings to the cbh1 promoter fragment (-304 bp to -18 bp) by electrophoresis mobility shift assays, suggesting ACEI and Xyr1 not only compete for binding to xyn1 promoter but also to cbh1 promoter.
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