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Updated: Jun 26, 2026

Undecalcified Bone Preparation for Histology, Histomorphometry and Fluorochrome Analysis
07:29

Undecalcified Bone Preparation for Histology, Histomorphometry and Fluorochrome Analysis

Published on: January 8, 2010

Staining undecalcified bone sections a modified technique for an improved visualization of synthetic bone substitutes

U Mayr-Wohlfart1, G Ravalli, K P Günther

  • 1Institute of Transfusion Medicine and Immunogenetics, Ulm, Germany.

Acta Chirurgiae Orthopaedicae Et Traumatologiae Cechoslovaca
|January 20, 2009
PubMed

Keywords:
bone histology techniquessynthetic bone graft analysisimproved staining methodsundecalcified bone sectioning

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Fixation and Sectioning01:03

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...