Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Disassembly of Intermediate Filaments

Disassembly of Intermediate Filaments

Intermediate filaments (IFs) do not undergo spontaneous disassembly. Enzymes, kinases, and phosphatases add and remove phosphates from specific sites to regulate their disassembly. The IF concentration in the cytoplasm also regulates the disassembly. If the concentration crosses a threshold, it activates the protein kinases in the vicinity, allowing the phosphorylation of IFs.
Keratin proteins, found at the cell periphery near cell junctions, undergo a cycle of assembly and disassembly. In Type...

Regulation of Nuclear Protein Sorting

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...

Nuclear Protein Sorting

Nuclear Protein Sorting

Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...

M-Cdk Drives Transition Into Mitosis

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...

M-Cdk Drives Transition Into Mitosis

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Modeling and decoupling phase-shift-induced wavefront distortion in high NA spherical Fizeau interferometry via aberration-related iterative algorithm.

Optics express·2026

Same author

DFT study of irradiation damage-defect correlations with mechanical properties in uranium nitride.

Scientific reports·2026

Same author

Dynamic calibration of the air-bearing table for the swing arm profilometer test.

Applied optics·2026

Same author

Millimeter-scale error correction with the frequency-adaptive dwell time optimization in spot-sized ion beam figuring.

Optics express·2026

Same author

Monocular Multi-Object 3D Visual Language Tracking.

IEEE transactions on image processing : a publication of the IEEE Signal Processing Society·2026

Same author

Design of highly efficient wideband multilayer coated blazed gratings providing constant <i>C<sub>ff</sub></i>-factor or deviation angle in the tender X-ray region.

Optics express·2025

Search research articles

Related Experiment Video

Updated: Jun 26, 2026

Live-imaging of Breast Epithelial Cell Migration After the Transient Depletion of TIP60

Live-imaging of Breast Epithelial Cell Migration After the Transient Depletion of TIP60

Published on: December 7, 2017

PML3 Orchestrates the Nuclear Dynamics and Function of TIP60.

Quan Wu¹, Haixiang Hu, Jianping Lan

¹Anhui Key Laboratory for Cellular Dynamics and Chemical Biology and National Laboratory for Physical Sciences at Nanoscale, Hefei 230027, China.

The Journal of Biological Chemistry

|January 20, 2009

Summary

The promyelocytic leukemia protein 3 (PML3) recruits TIP60 to nuclear bodies, protecting it from degradation and ensuring genomic stability. This interaction is crucial for DNA repair processes.

More Related Videos

Chemical Dimerization-Induced Protein Condensates on Telomeres

Chemical Dimerization-Induced Protein Condensates on Telomeres

Published on: April 12, 2021

Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion

Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion

Published on: July 27, 2021

Related Experiment Videos

Last Updated: Jun 26, 2026

Live-imaging of Breast Epithelial Cell Migration After the Transient Depletion of TIP60

Live-imaging of Breast Epithelial Cell Migration After the Transient Depletion of TIP60

Published on: December 7, 2017

Chemical Dimerization-Induced Protein Condensates on Telomeres

Chemical Dimerization-Induced Protein Condensates on Telomeres

Published on: April 12, 2021

Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion

Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion

Published on: July 27, 2021

Area of Science:

Cell Biology
Molecular Biology
Genetics

Background:

The promyelocytic leukemia (PML) protein is essential for PML nuclear body (NB) assembly and function.
PML NBs recruit sumoylated proteins, but the regulatory mechanism is not fully understood.

Purpose of the Study:

To investigate the mechanism by which PML protein regulates protein recruitment to NBs.
To elucidate the specific role of PML3 isoform in this process.

Main Methods:

Biochemical characterization of PML3-TIP60 interaction.
Fluorescence recovery after photobleaching (FRAP) analysis.
Studies in PML3-deficient cells.

Main Results:

PML3 specifically interacts with and recruits TIP60 to PML NBs via its N-terminal region.
This interaction protects TIP60 from Mdm2-mediated degradation.
PML3-TIP60 interaction modulates TIP60's distribution, mobility, and function in DNA damage response.

Conclusions:

PML3 orchestrates the distribution, dynamics, and function of TIP60.
The PML3-TIP60 interaction is a novel regulatory mechanism crucial for maintaining genomic stability.