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Updated: Jan 13, 2026

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Surface plasmon resonance biosensor for biomolecular interaction analysis based on spatial modulation phase

Xiang Ding¹, Fangfang Liu, Xinglong Yu

¹Department of Precision Instruments and Mechanics, Tsinghua University, Beijing, China.

Methods in Molecular Biology (Clifton, N.J.)

|January 20, 2009

Summary

This summary is machine-generated.

Spatial modulation phase detection enhances surface plasmon resonance (SPR) biosensors for analyzing small molecules. This advanced technique offers greater sensitivity than traditional intensity detection for biomolecular interaction analysis.

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Last Updated: Jan 13, 2026

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Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance SPR

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance SPR

Published on: November 29, 2014

Area of Science:

Biomedical Engineering
Analytical Chemistry
Biophysics

Background:

Surface plasmon resonance (SPR) biosensors are vital for biomolecular interaction analysis in proteomics and drug discovery.
Current SPR methods require enhanced sensitivity for analyzing small molecules.

Purpose of the Study:

To develop and evaluate a novel SPR biosensor system utilizing spatial modulation phase detection (SMPD) for improved sensitivity.
To demonstrate the capability of SMPD-based SPR for array detection and kinetic analysis.

Main Methods:

An SPR system was developed employing spatial modulation phase detection (SMPD) with collimated monochromatic light.
The system generates an interference pattern by spatially modulating the phase of reflected light.
Algorithms process the interference pattern to obtain instantaneous phase distribution for real-time biomolecular interaction monitoring.

Main Results:

The developed SPR system successfully performed array detection using an antibody array chip.
Phase detection demonstrated higher sensitivity compared to traditional intensity detection methods.
The system enables continuous monitoring of biomolecular interactions and kinetic analysis.

Conclusions:

SMPD-based SPR biosensing offers a significant advancement in sensitivity for small molecule analysis.
This technique is suitable for array-based detection and provides valuable kinetic parameters.
The developed system holds promise for applications in proteomics and drug discovery research.