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Related Experiment Video

Updated: Jun 26, 2026

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)
09:35

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)

Published on: November 29, 2014

Surface plasmon resonance biosensing.

Marek Piliarik1, Hana Vaisocherová, Jirí Homola

  • 1Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic, Prague, Czech Republic.

Methods in Molecular Biology (Clifton, N.J.)
|January 20, 2009
PubMed
Summary
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Surface plasmon resonance (SPR) biosensors are label-free optical tools. They detect biomolecular interactions and analytes for medical, environmental, and food safety applications.

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Biosensing Technologies

Background:

  • Surface plasmon resonance (SPR) biosensors are label-free optical devices.
  • They measure refractive index changes caused by analyte binding to immobilized bioreceptors.
  • SPR biosensors have been pivotal in life science and pharmaceutical research since the late 1990s.

Purpose of the Study:

  • To review the fundamental principles of SPR biosensor technology.
  • To discuss the diverse applications of SPR biosensors.
  • To highlight the technology's utility in rapid, sensitive, and specific analyte detection.

Main Methods:

  • Optical measurement of refractive index changes.
  • Utilizing biorecognition molecules immobilized on SPR sensors.

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A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
09:09

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Related Experiment Videos

Last Updated: Jun 26, 2026

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)
09:35

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)

Published on: November 29, 2014

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
09:09

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

  • Label-free detection of analyte binding events.

    • Main Results:

    • SPR biosensors enable label-free detection of biomolecular interactions.
    • The technology is increasingly applied in medical diagnostics, environmental monitoring, and food safety.
    • SPR biosensors offer rapid, sensitive, and specific detection capabilities for various analytes.

    Conclusions:

    • SPR biosensor technology is a versatile tool for studying biomolecular interactions.
    • Its applications extend to critical areas like diagnostics, environmental monitoring, and food safety.
    • The technology provides a label-free, sensitive, and specific method for analyte detection.