Related Experiment Video
Updated: Jun 26, 2026
Routine Screening Method for Microparticles in Platelet Transfusions
Published on: January 31, 2018
Accumulation of soluble inflammatory mediators between blood donation and pre-storage leucocyte depletion
D Chudziak1, W Sireis, H-U Pfeiffer
1German Red Cross Blood Centre and Institute for Transfusion Medicine and Immunohaematology of the Johann-Wolfgang-Goethe University, Frankfurt, Germany.
Background And Objectives:
Leucocyte-derived cytokines accumulate in stored whole blood. Pre-storage leucocyte depletion has reduced cytokine levels and, consequently, febrile non-haemolytic transfusion reactions. As leucocyte filtration and component separation can be performed until 24 h after donation, we hypothesized that within this time, inflammatory cytokines might accumulate.
Materials And Methods:
Serial plasma samples were collected 4, 10 and 20 h after donation and cytokine concentrations were measured.
Results:
Interleukin-8 increased > 20-fold and soluble CD40 ligand > sixfold during the observation time, less pronounced changes for several other mediators were also observed.
Conclusion:
Leucocyte depletion within 10 h of blood donation will reduce the concentrations of pyrogenic mediators.
