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Updated: Jun 26, 2026

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

Instrumentation for fluorescence-based fiber optic biosensors.

Richard B Thompson¹, Hui-Hui Zeng, Daniel Ohnemus

¹Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA.

Methods in Enzymology

|January 21, 2009

Summary

This summary is machine-generated.

This chapter details fluorescence-based fiber optic sensors for chemical analysis. It covers sensor construction, operation, and calibration, including metal ion determination using fluorescence changes.

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Area of Science:

Analytical Chemistry
Optical Sensing Technologies

Background:

Fiber optic sensors offer remote chemical detection capabilities.
Fluorescence-based transduction provides sensitive analyte recognition.

Purpose of the Study:

To summarize the principles, operation, and calibration of single-fiber fluorescence-based fiber optic sensors.
To provide examples of metal ion determination in aqueous solutions.

Main Methods:

Discusses sensor construction and operational principles.
Details calibration procedures and instrument alignment.
Covers noise source identification and probe preparation.

Main Results:

Demonstrates remote measurement of chemical analytes via fluorescence changes (wavelength or lifetime).
Provides examples of metal ion determination using fluorescence ratio and lifetime techniques.

Conclusions:

Fluorescence-based fiber optic sensors are versatile tools for remote chemical sensing.
Practical considerations for field and laboratory use are addressed, including long fiber applications.