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Updated: Jun 26, 2026
Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis
Published on: September 7, 2021
Embryo quality and implantation rate in two different culture media: ISM1 versus Universal IVF Medium
Susanna Xella1, Tiziana Marsella, Daniela Tagliasacchi
1Mother-Infant Department, Institute of Obstetrics and Gynaecology, University of Modena and Reggio Emilia, Modena, Italy. susannaxella@yahoo.it
Objective:
To compare the outcome of two different culture media marketed by the MediCult AS Company (Jyllinge, Denmark)-Universal IVF Medium and ISM1 Medium culture-which, in addition to glucose, pyruvate, and energy-providing components, also contain amino acids, nucleotides, vitamins, and cholesterol.
Design:
Laboratory and retrospective clinical study.
Setting:
University teaching hospital.
Patient(S):
A total of 726 patients, undergoing IVF-intracytoplasmic sperm injection procedure, comparable in mean age range, oocyte retrieval, and infertility indication, were included in the study. Laboratory quality and standard procedures were maintained unaffected.
Intervention(S):
Oocyte retrieval, different embryo culture media.
Main Outcome Measure(S):
Embryo quality, ongoing pregnancy, and implantation rate.
Result(S):
The frequency of good-quality embryos (79% vs. 74%) and the percentages of ongoing pregnancy (27.5% vs. 18%) and implantation rate (15% vs. 10%) were significantly higher in the group treated with ISM1 Medium rather than Universal IVF Medium.
Conclusion(S):
ISM1 Medium culture seems to improve the performance of embryonic growth and development, as well as increasing the percentage of pregnancy.
