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Related Experiment Videos

A rearranged lambda 2 light gene chain retards but does not exclude kappa and lambda 1 expression.

B Bogen1, S Weiss

  • 1Institute of Immunology and Rheumatology, University of Oslo, Norway.

European Journal of Immunology
|October 1, 1991
PubMed
Summary
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In MOPC315 transgenic mice, lambda 2 light chain expression on B cells is initially exclusive but becomes leaky with age. Antigen exposure favors a specific B cell population, influencing immunoglobulin production in adult mice.

Area of Science:

  • Immunology
  • Molecular Biology

Background:

  • Mice were engineered to express the MOPC315 lambda 2 light chain.
  • Isotypic exclusion, the suppression of other immunoglobulin light chains, is a key aspect of B cell development.

Purpose of the Study:

  • To investigate the developmental regulation of immunoglobulin light chain expression in transgenic mice.
  • To understand the impact of lambda 2 expression on B cell populations and serum immunoglobulin composition over time.

Main Methods:

  • Generation of transgenic mice expressing the MOPC315 lambda 2 light chain.
  • Analysis of B cell populations using flow cytometry.
  • Characterization of serum immunoglobulins and B cell hybridomas.

Main Results:

Related Experiment Videos

  • Newborn transgenic mice showed exclusive lambda 2 expression on B cells.
  • By 21 days, double expresser populations (lambda 2/kappa and lambda 2/lambda 1) emerged.
  • Serum immunoglobulin analysis revealed a decline in lambda 2 fraction with age and increased co-expression of kappa and lambda 1.
  • Adult B cell hybridomas frequently secreted mixed lambda 2/kappa molecules and showed kappa gene rearrangement.

    • Conclusions:

    • Isotypic exclusion of lambda 2 is complete at birth but becomes leaky with age in transgenic mice.
    • Antigen likely expands the lambda 2 low kappa high B cell population, which is a major source of serum Ig in adults.
    • The lambda 2 high kappa low population, often CD5+, appears less involved in antibody secretion in adult transgenic mice.