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Published on: October 1, 2007
A multi-purpose microfluidic perfusion system with combinatorial choice of inputs, mixtures, gradient patterns, and
Gregory A Cooksey1, Christopher G Sip, Albert Folch
1Department of Bioengineering, University of Washington, Seattle, WA 98195, USA.
Lab on a Chip
|January 22, 2009
Summary
This study introduces a versatile microfluidic perfusion device for advanced cell culture. The system offers precise control over fluid flow, enabling complex gradient generation for diverse biological applications.
Area of Science:
- Biotechnology
- Microfluidics
- Cell Biology
Background:
- Microfluidic perfusion systems offer advantages like low reagent consumption and high integration.
- These systems are increasingly vital for cell culture studies in various biological fields.
Purpose of the Study:
- To develop a multipurpose, computer-controlled microfluidic perfusion device.
- To enable precise control over fluid flow dynamics and gradient generation within a cell culture chamber.
Main Methods:
- The device features sixteen inlets and a large cell culture chamber.
- It utilizes a pressure-driven system with a binary multiplexer for inlet selection and control.
- Functionalities include bypass, heterogeneous laminar flow, gradient formation, homogenization, and flow rate modulation.
Main Results:
- The system allows for sub-second temporal resolution in selecting inlets and controlling flow.
- Complex gradient patterns and sequential perfusions can be generated within the chamber.
- Asymmetrical and curvilinear flow patterns are achievable through side outlet manipulation.
Conclusions:
- The developed microfluidic device provides a versatile platform for sophisticated cell culture experiments.
- Its advanced fluid control capabilities support applications in tissue engineering, systems biology, and molecular assays.
