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Related Concept Videos

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Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
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The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...

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Related Experiment Video

Updated: Jun 26, 2026

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Enhanced on-chip SERS based biomolecular detection using electrokinetically active microwells.

Yun Suk Huh1, Aram J Chung, Bernardo Cordovez

  • 1Sibley School of Mechanical and Aerospace Engineering, Cornell University, Ithaca, NY 14853, USA. de54@cornell.edu.

Lab on a Chip
|January 22, 2009
PubMed
Summary
This summary is machine-generated.

This study introduces a new microfluidic chip for sensitive and specific Dengue virus detection using surface-enhanced Raman spectroscopy (SERS). The technique actively concentrates and mixes samples for enhanced biomolecular detection.

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Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Microfluidics

Background:

  • Developing sensitive and specific methods for pathogen detection is crucial for public health.
  • Existing on-chip biomolecular detection techniques have limitations in sample handling and detection sensitivity.
  • Surface-enhanced Raman spectroscopy (SERS) offers high sensitivity but requires efficient sample preparation.

Purpose of the Study:

  • To present a novel microfluidic technique for on-chip SERS-based biomolecular detection.
  • To combine advantages of homogeneous and heterogeneous on-chip detection methods.
  • To demonstrate the technique's application in quantitative detection of Dengue virus sequences.

Main Methods:

  • Fabrication of a microfluidic chip with electrokinetically active microwells.
  • Utilizing electrokinetic effects for active mixing and concentration of analytes.
  • Applying the chip for quantitative SERS detection of nucleic acid sequences.
  • Numerical analysis of fluid flow patterns within the microfluidic device.

Main Results:

  • Demonstrated active mixing to enhance binding rates between SERS enhancers and targets.
  • Achieved rapid concentration of detection products for optical interrogation.
  • Reported a limit of detection of 30 pM for Dengue virus serotype 2 sequences.
  • Showcased excellent specificity against other Dengue virus serotypes.

Conclusions:

  • The developed microfluidic SERS technique enables efficient sample manipulation for enhanced biomolecular detection.
  • The device offers a sensitive and specific platform for quantitative detection of viral nucleic acids.
  • This approach holds promise for rapid and accurate point-of-care diagnostics.