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Electrolyte systems for on-line CE-MS: detection requirements and separation possibilities
Pavla Pantůcková1, Petr Gebauer, Petr Bocek
1Institute of Analytical Chemistry, ASCR, Brno, Czech Republic.
Electrophoresis
|January 22, 2009
Summary
This review details electrolyte systems for on-line capillary electrophoresis-mass spectrometry (CE-MS), highlighting their pros and cons for effective separation and identification of analytes.
Area of Science:
- Analytical Chemistry
- Separation Science
Background:
- On-line capillary electrophoresis-mass spectrometry (CE-MS) is a powerful analytical tool with established instrumentation.
- The focus is shifting towards optimizing the chemical (wet, electrophoretic) aspects for method success.
- Electrolyte systems are crucial for achieving effective separation in CE-MS.
Purpose of the Study:
- To review electrolyte systems used in the capillary electrophoresis (CE) component of CE-MS.
- To provide an in-depth analysis of the advantages and disadvantages of various electrolyte systems.
- To discuss advanced electrophoretic techniques and their application in CE-MS.
Main Methods:
- Comprehensive literature review of electrolyte systems in CE-MS.
- Analysis of over 200 selected applications, categorized by analyte.
- Discussion of advanced techniques like sample stacking and non-aqueous solvents.
Main Results:
- Detailed overview of electrolyte systems employed in CE-MS applications.
- Evaluation of the strengths and weaknesses of different electrolyte choices.
- Insights into the practical implementation of various electrophoretic approaches.
Conclusions:
- The selection of appropriate electrolyte systems is critical for successful CE-MS method development.
- Advanced electrophoretic techniques offer enhanced separation capabilities for complex samples.
- This review serves as a guide for researchers in optimizing CE-MS for specific analytes.
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