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18-crown-6 as a class I organic modifier in MEKC
Raychelle M Burks1, David S Hage
1Department of Chemistry, University of Nebraska, Lincoln, NE 68588-0304, USA.
This study introduces 18-crown-6 as an organic modifier for sodium dodecyl sulfate micellar electrokinetic chromatography (SDS MEKC). It reveals how 18-crown-6 influences the separation of neutral and anionic compounds.
Area of Science:
- Analytical Chemistry
- Separation Science
- Electrochemistry
Background:
- Micellar electrokinetic chromatography (MEKC) is a powerful separation technique.
- Organic modifiers are crucial for optimizing MEKC selectivity.
- 18-crown-6 is investigated as a novel modifier.
Purpose of the Study:
- To evaluate 18-crown-6 as a class I organic modifier in SDS MEKC.
- To investigate the impact of 18-crown-6 on analyte retention for different compound classes.
Main Methods:
- Sodium dodecyl sulfate micellar electrokinetic chromatography (SDS MEKC) was employed.
- Two series of positional isomers were analyzed: neutral nitrotoluenes and anionic nitrophenols.
- The effect of 18-crown-6 concentration on separation was studied.
Main Results:
- 18-crown-6 demonstrated differential effects on the retention of neutral and anionic analytes.
- Analyte retention in SDS MEKC was modulated by the presence of 18-crown-6.
- Positional isomers showed distinct responses to the modifier.
Conclusions:
- 18-crown-6 is effective as a class I organic modifier in SDS MEKC.
- The modifier offers a means to control selectivity for various analyte types.
- This work expands the utility of organic modifiers in MEKC separations.
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