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Published on: April 28, 2011
Determining the conformational stability of a protein using urea denaturation curves
Kevin L Shaw1, J Martin Scholtz, C Nick Pace
1Department of Biology, Grove City College, Grove City, PA, USA.
Abstract:
The stability of globular proteins is an important factor in determining their usefulness in basic research and medicine. A number of environmental factors contribute to the conformational stability of a protein, including pH, temperature, and ionic strength. In addition, variants of proteins may show remarkable differences in stability from their wild-type form. In this chapter, we describe the method and analysis of urea denaturation curves to determine the conformational stability of a protein. This involves relatively simple experiments that can be done in a typical biochemistry laboratory, especially when using ordinary spectroscopic techniques to follow unfolding.
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