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Updated: Jun 26, 2026

HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis
Published on: November 11, 2022
[Study on fingerprints of Citrus aurantium from different places by capillary electrophoresis]
Guang-Ming Luo1, Xiao-Hui Tan, Li-Fang Xu
1Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China. jzlgm11@163.com
Objective:
To develop a high performance capillary electrophoresis method for Citrus aurantium fingerprints to control its quality.
Method:
The background electrolyte (BGE) was an 80 mmol x L(-1) boric acid solution containing 15 mmol x L(-1) borate. The pH of the BGE was adjusted to 9.70 with KOH solution. The detection wavelength was 201 nm and a voltage of 16 kV was applied. The sample hydrodynamic injection was 0.4 ps with a duration time of 8 sec. C. aurantium was extracted by water and a set of capillary electrophoresis fingerprints (CEFP) containing 12 co-possessing peaks was obtained.
Result:
There were good similarities between the standard CEFP and each set of CEFP of C. aurantium collected from eleven different places, and their similarity coefficients were between 0.973 and 0.996.
Conclusion:
The CEFP has acceptable precision, reproducibility and stability and can be used for the quality control of C. aurantium.
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