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Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
Published on: December 29, 2015
Nucleotide sequence requirements at the 5' end of the influenza A virus M RNA segment for efficient virus replication
Makoto Ozawa1, Junko Maeda, Kiyoko Iwatsuki-Horimoto
1Division of Virology, Department of Microbiology and Immunology, University of Tokyo, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
Abstract:
The mechanism by which the influenza A virus genome is packaged into virions is not fully understood. The coding and noncoding regions necessary for packaging of the viral RNA segments, except for the M segment, have been identified. Here, we delineate the M segment regions by incorporating a reporter viral RNA into virions and by generating viruses possessing mutations in the regions. We found that, like the other segments, the M segment coding regions are essential for virion incorporation and that the nucleotide length rather than the nucleotide sequence of the 5' end of the coding region is important.
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