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The Pro117 to glycine mutation of staphylococcal nuclease simplifies the unfolding-folding kinetics
K Kuwajima1, N Okayama, K Yamamoto
1Department of Polymer Science, Faculty of Science, Hokkaido University, Japan.
Abstract:
Kinetics of unfolding and refolding of a staphylococcal nuclease mutant, in which Pro117 is replaced by glycine, have been investigated by stopped-flow circular dichroism, and the results are compared with those for the wild-type protein. In contrast to the biphasic unfolding of the wild-type nuclease, the unfolding of the mutant is represented by a single-phase reaction, indicating that the biphasic unfolding for the wild-type protein is caused by cis-trans isomerization about the prolyl peptide bond in the native state. The proline mutation also simplifies the kinetic refolding. Importance of the results in elucidating the folding mechanism is discussed.
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