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Overproduction, purification and crystallization of Bacillus cereus oligo-1,6-glucosidase
K Watanabe1, K Kitamura, Y Hata
1Department of Agricultural Chemistry, Kyoto Prefectural University, Japan.
FEBS Letters
|September 23, 1991
Abstract:
The gene coding for oligo-1,6-glucosidase from Bacillus cereus ATCC7064 has been overexpressed in Escherichia coli MV1184 cells under the control of the lac promoter in the genetically engineered plasmid pBCE4-2. Oligo-1,6-glucosidase was purified in large quantities and was crystallized at 25 degrees C by using a hanging drop vapor diffusion method with 53% saturated ammonium sulfate. The crystals have the shape of hexagonal bipyramids and belong to the space group P6(2) or P6(4) with lattice constants of a = b = 106.1 A, c = 120.0 A and gamma = 120 degrees.