Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

776
Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
776
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

839
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
839
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

1.8K
When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
1.8K
¹H NMR: Complex Splitting01:13

¹H NMR: Complex Splitting

2.1K
A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied...
2.1K
2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

889
Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other...
889
NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

1.1K
When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
1.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Coarse-Grained Free Energy Surface with Implicit Nuclear Quantum Effects: Benzene Clusters.

The journal of physical chemistry. A·2026
Same author

p-H2 vs o-D2 clusters: From liquid-like to glass-like behavior.

The Journal of chemical physics·2026
Same author

Analysis of Food Contact Paper by Hydrolysis and <sup>19</sup>F-NMR.

Analytical chemistry·2026
Same author

Validating Direct Mass Spectrometry Screening for Grease-Proofers Containing 6:2 Fluorotelomer Alcohol in Fiber-Based Food Packaging.

Journal of agricultural and food chemistry·2026
Same author

Identification and quantification of a sulphonated quinolinecarboxylic acid in the color additive D&C Yellow No. 10 (Quinoline Yellow).

Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment·2026
Same author

Determination of Subsidiary Colors in D&C Red No. 36 (Pigment Red 4) by HPLC and UHPLC.

Journal of AOAC International·2025
Same journal

pyTRACTnmr: an open source python package for analyzing [<sup>15</sup>N, <sup>1</sup>H]-TRACT experiments.

Journal of biomolecular NMR·2026
Same journal

RelCalc: symbolic evaluation of BWR theory relaxation rates in python, applications to TROSY effects in AX[Formula: see text] spin systems.

Journal of biomolecular NMR·2026
Same journal

Solution NMR study of the titin I-band IgI domain I82 shows unusual conformational dynamics.

Journal of biomolecular NMR·2026
Same journal

Methyl-specific NMR of therapeutic antibodies: cost-effective isotopic labeling strategies in CHO cells for high-resolution structural characterization.

Journal of biomolecular NMR·2026
Same journal

AMIGO - Guided assignment of <sup>13</sup>C-methyl labelled proteins.

Journal of biomolecular NMR·2026
Same journal

Super-Resolution solid-state NMR Spectroscopy.

Journal of biomolecular NMR·2026
See all related articles

Related Experiment Video

Updated: Mar 24, 2026

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
07:24

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

Published on: September 23, 2021

2.3K

On projection-reconstruction NMR.

Clark D Ridge1, Vladimir A Mandelshtam

  • 1Chemistry Department, University of California at Irvine, Irvine, CA 92697, USA. mandelsh@uci.edu

Journal of Biomolecular NMR
|January 23, 2009
PubMed
Summary
This summary is machine-generated.

This study evaluates simple projection-reconstruction algorithms for spectral data. A novel histogram-based method shows promise in handling noisy spectra with overlapping or opposite-phase peaks, potentially reducing data requirements.

More Related Videos

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

16.2K
Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo
08:01

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo

Published on: September 26, 2016

9.9K

Related Experiment Videos

Last Updated: Mar 24, 2026

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
07:24

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

Published on: September 23, 2021

2.3K
Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

16.2K
Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo
08:01

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo

Published on: September 26, 2016

9.9K

Area of Science:

  • Signal Processing
  • Spectroscopy
  • Image Reconstruction

Background:

  • Traditional projection-reconstruction algorithms face challenges with noisy spectral data and overlapping peaks.
  • Existing methods may struggle with spectra containing peaks of opposite phase, limiting their applicability.

Purpose of the Study:

  • To analyze the performance of simple projection-reconstruction algorithms.
  • To introduce and explore a new histogram-based algorithm for spectral reconstruction.
  • To compare the new algorithm against existing methods, particularly in challenging spectral conditions.

Main Methods:

  • Analysis of three established algorithms: Lowest-Value, Additive Back-Projection, and Hybrid Back-Projection/Lowest-Value.
  • Development and testing of a novel algorithm utilizing amplitude histograms for spectral reconstruction.
  • Validation using simulated spectral data to assess performance under various conditions.

Main Results:

  • All evaluated algorithms offer potential data reduction for spectral reconstruction.
  • Existing algorithms demonstrate limitations with noisy spectra and overlapping peaks.
  • The new histogram-based algorithm shows improved capability in handling spectra with opposite-phase peaks.

Conclusions:

  • Simple projection-reconstruction algorithms can reduce data needs but have limitations.
  • The novel histogram-based algorithm presents an advantage for spectral reconstruction, especially with complex peak structures.
  • Further research into histogram-based methods could enhance spectral analysis robustness.