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A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes
Published on: June 10, 2022
Flow injection based methods for fast screening of antioxidant capacity.
Luís M Magalhães1, Miguel Santos, Marcela A Segundo
1REQUIMTE, Serviço de Química-Física, Faculdade de Farmácia, Universidade do Porto, Rua Aníbal Cunha, 164, 4099-030 Porto, Portugal.
Talanta
|January 23, 2009
Summary
Automated antioxidant capacity analysis is crucial for various fields. This review compares flow injection methods using ABTS(+) or DDPH radicals and reducing capacity assays for efficiency and real-world application.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Food Science
Background:
- Antioxidants play vital roles in physiology and food technology.
- Accurate analytical methods are essential for quantifying antioxidant properties.
- Automation of antioxidant capacity determination is increasingly important for routine analysis.
Purpose of the Study:
- To critically compare various automated flow injection analysis (FIA) methods for determining antioxidant capacity.
- To evaluate the performance of these methods against traditional batch procedures.
- To assess the applicability of these automated methods to real-world samples.
Main Methods:
- Review of existing literature on flow injection analysis (FIA) methods for antioxidant capacity.
- Comparison of methods based on scavenging of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulphonate) radical cation (ABTS(+)).
- Comparison of methods based on scavenging of 2,2-diphenyl-1-picrylhydrazyl radical (DDPH) or total reducing capacity determination.
Main Results:
- Several FIA methods have been proposed for antioxidant capacity determination.
- The review assesses the degree of automation achieved by different methods.
- Performance and applicability to real samples are key comparison criteria.
Conclusions:
- Automated antioxidant capacity assays offer potential for routine screening.
- Critical comparison of FIA methods is needed to guide selection for specific applications.
- Further validation is required for widespread adoption in diverse fields.
