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[Effect of selenium-protein polysaccharide extracted from Se-rich Cordyceps militaris on tumor-bearing mice]
Ming Zhong1, Li-He Wang, Hui Ma
1Key Laboratory of Agriculture Biotechnology of Liaoning Province, Shenyang Agricultural University, Shenyang 110161, China. zhming@syau.edu.cn
Objective:
To extract and analysis the active components of Se-protein polysaccharide from Se-rich Cordyceps militaris, and to discuss the anti-tumor effect of Se-protein polysaccharide.
Method:
Protein, polysaccharides and selenium content were determined by the methods of Folin-phenol reagent (lowry), phenol-sulfate and DAN fluorescence, respectively. Tumor-bearing mouse model was established and divided into the model group, cyclophosphamide group, cordyceps high and low dosage group (200, 100 mg x kg(-1)). Then the Se-protein polysaccharide's anti-tumor activity and immune function in vivo were observed by compare with model group in the weight of mice, inhibitory rate, conversion rate of peripheral blood lymphocytes, dissection index K, swallowed factor alpha, liver and spleen factor coefficient, GSH-Px and SOD activity and the content of MDA.
Result:
Se-protein polysaccharides from Se-rich Cordyceps militaris had a significant anti-tumor action with the inhibitory rate 46.92% and could avoid toxic effect of chemotherapy drug like cyclophosphamide. It also could enhance immune function and body antioxidant capacity by inhibiting the decline of tumor-bearing mouse liver coefficient and spleen coefficient in tumor-bearing mice caused by cyclophosphamide.
Conclusion:
Se-protein polysaccharide, the extraction of Se-rich Cordyceps militaris's can inhibit tumor grouth of tumor-bearing mouse.