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Updated: Jun 26, 2026

Mouse Genome Engineering Using Designer Nucleases

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

SNAP-25 and gene-targeted mouse mutants.

Christina Bark¹

¹The Rolf Luft Center for Diabetes and Endocrinology, Department of Molecular Medicine and Surgery, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden. Christina.Bark@ki.se

Annals of the New York Academy of Sciences

|January 24, 2009

Summary

The SNARE fusion machinery releases neurotransmitters and hormones. Two SNAP-25 protein variants, SNAP-25a and SNAP-25b, are generated by alternative splicing, and balanced expression is crucial for physiological function.

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Area of Science:

Molecular Biology
Neuroscience
Cell Biology

Background:

The N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) machinery mediates neurotransmitter and hormone release.
SNAP-25 (synaptosomal-associated protein of 25 kD) is a key component, localized to the plasma membrane with syntaxin 1.
VAMP/synaptobrevin is associated with secretory vesicles, working with SNAREs and accessory factors for vesicle docking, priming, and fusion.

Purpose of the Study:

To investigate the biological relevance of the two SNAP-25 protein variants, SNAP-25a and SNAP-25b.
To understand the developmental and neuroanatomical regulation of SNAP-25 variant expression.
To clarify the importance of balanced SNAP-25a and SNAP-25b expression for physiological processes.

Main Methods:

Analysis of gene-targeted mouse mutants.
Studying the alternative splicing mechanism generating SNAP-25a and SNAP-25b from a single gene.
Investigating the role of exon 5 in posttranslational palmitoylation and membrane anchoring.

Main Results:

SNAP-25a and SNAP-25b are generated by alternative splicing of exon 5, which is involved in membrane anchoring.
Alternative splicing is developmentally and neuroanatomically regulated.
Gene-targeted mouse mutants highlight the importance of maintaining physiological total SNAP-25 levels with balanced SNAP-25a and SNAP-25b expression.

Conclusions:

Balanced expression of SNAP-25a and SNAP-25b is physiologically important.
The regulation of SNAP-25 variant expression plays a critical role in cellular functions.
Further research is needed to fully elucidate the functional significance of these distinct SNAP-25 variants.