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Polyelectrolyte microcapsule interactions with cells in two- and three-dimensional culture.

Zhihua An1, Krithika Kavanoor, Megan L Choy

  • 1Department of Chemistry, Columbia University, 3000 Broadway, New York, NY 10027, USA.

Colloids and Surfaces. B, Biointerfaces
|January 24, 2009
PubMed
Summary

Poly-(sodium 4-styrene sulfonate) [PSS] and poly-(allylamine hydrochloride) [PAH] microcapsules show good biocompatibility in cell culture and 3D matrices. These microcapsules are suitable for drug delivery research, demonstrating minimal impact on cell viability and tumor spheroid growth.

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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Cell Biology

Background:

  • Layer-by-layer self-assembled microcapsules offer tunable physicochemical properties for advanced applications.
  • Biocompatibility is a critical factor for the successful translation of microcapsule-based drug delivery systems.
  • Understanding cellular interactions with microcapsules in both 2D and 3D environments is essential.

Purpose of the Study:

  • To evaluate the biocompatibility of poly-(sodium 4-styrene sulfonate) [PSS]/poly-(allylamine hydrochloride) [PAH] microcapsules.
  • To compare the biocompatibility of PSS/PAH microcapsules with other polyelectrolyte microcapsules.
  • To assess the impact of PSS/PAH microcapsules on cell behavior in 2D cultures and 3D tumor models.

Main Methods:

  • Co-culture of C6 glioma and 3T3 fibroblast cells with PSS/PAH microcapsules on 2D substrates.

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  • Culturing cells with various polyelectrolyte microcapsules to compare biocompatibility.
  • Embedding cells within 3D collagen matrices and evaluating microcapsule effects on invasion and spheroid growth.

    • Main Results:

    • PSS/PAH microcapsules exhibited comparable biocompatibility to other microcapsules at high ratios (up to 100:1).
    • Cellular responses varied with microcapsule composition, suggesting specific biochemical interactions.
    • High concentrations of PSS/PAH microcapsules showed negligible effects on cell invasion and tumor spheroid growth in 3D matrices.

    Conclusions:

    • PSS/PAH microcapsules demonstrate sufficient biocompatibility for in vitro drug delivery studies.
    • Biochemical interactions, not mechanical effects, appear to drive cellular responses to microcapsules.
    • These microcapsules show promise as drug delivery agents in simulated tumor microenvironments.