Passaged neural stem cell-derived neuronal networks for a portable biosensor.

Area of Science:

• Neuroscience
• Biotechnology
• Sensor Technology

Background:

• Portable biosensors previously used short-lived primary neuronal cultures on microelectrode arrays (MEAs).
• A renewable source of neuronal networks is needed to extend biosensor shelf life for fieldable applications.

Purpose of the Study:

• To develop a strategy for culturing passaged neural stem and progenitor cells on MEAs.
• To produce differentiated neurons and glia forming functional neuronal networks for biosensor applications.

Main Methods:

• Neuroepithelial stem and progenitor cells from embryonic rat cortex were seeded on MEAs.
• Cells were cultured in serum-free medium with bFGF and BDNF.
• Neuronal network function was assessed via action potential recording and drug response.

Main Results:

• Passaged neural stem and progenitor cells differentiated into functional neuronal networks with neurons and astrocytes.
• Spontaneous action potentials were recorded 4-5 weeks post-culture.
• Networks responded to GABA(A) and glutamate receptor antagonists, indicating active synapses.

Conclusions:

• Passaged neural stem and progenitor cells provide a renewable source for neuronal networks on MEAs.
• These networks exhibit properties similar to primary neuronal cultures.
• This approach enables a sustainable supply of sensor elements for detecting environmental threats.