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[A simple method of separating outer hair cells in Guinea pig]
Xixun Kang1, Weijia Kong, Wanrong Li
1Department of Otolaryngology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Objective:
In order to meet the needs of electrophysiology outer hair cells, we investigate a method to separate outer hair cells simply and effectively.
Method:
The basal membrane was dissected combined with spiral ligament and axis of cochlea, then incubated with enzyme, and then mechanically triturated by micropipette.
Result:
A large number of living outer hair cells were obtained. Of 80% cells could keep good condition in 6 hours.
Conclusion:
If increasing the enzyme concentration slightly and the large part of cochlea without outer bone was enzymes, we can get living outer hair cells easily.
