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The cytotoxicity of self-etching primer bonding agents in vitro
La-ongthong Vajrabhaya1, Suwanna Korsuwannawong, Claudia Bosl
1Department of Operative Dentistry, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.
Objective:
This study evaluated the cytotoxicity of 3 self-etching bonding systems: Optibond Solo Plus SE primer, Xeno III, and i Bond.
Study Design:
The test materials were applied on the dentine discs of dentine barrier models in the same way as in the clinical procedures recommended by each manufacturer. 3-D cell culture of Bovine pulp-derived cells transfected with Simian virus 40 Large T antigen and perfusion condition were conducted in this experiment. Cell viability after exposure to the bonding agents was determined by dimethylthiazolediphenyltetrazolium bromide (MTT) assay.
Results:
The results revealed that cell survival with the above-mentioned bonding agents was 99.66%, 72.59%, and 10.65%, respectively. i Bond is the most toxic material (P < .05). Xeno III is less toxic than i Bond but more toxic than Optibond Solo Plus SE primer (P < .05).
Conclusions:
Among the 3 test self-etching bonding systems, Optibond Solo Plus SE primer should be selected in cases where the remaining dentin above pulp tissue is 0.5 mm or less.
