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Updated: Jun 26, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
Published on: April 4, 2025
Protein recognition by an ensemble of fluorescent DNA G-quadruplexes
David Margulies1, Andrew D Hamilton
1Department of Chemistry, Yale University, P. O. Box 208107, New Haven, CT 06520-8107, USA.
Abstract:
Sniffing out proteins: Fluorescent DNA G-quadruplexes have been used for building versatile signaling receptors for proteins in a single solution. Introducing a protein sample to the ensemble results in a unique emission signature for unambiguous identification (see scheme, R = fluorophore). The self-assembled, pattern-based protein detection systems are easily fabricated, have the potential for high-throughput operations, and have the ability to handle small protein samples.

