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Related Concept Videos

Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Mass Analyzers: Overview01:13

Mass Analyzers: Overview

The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
Mass Spectrometers01:16

Mass Spectrometers

This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
Mass Spectrum: Interpretation01:24

Mass Spectrum: Interpretation

An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a soft-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.To...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...

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T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis
16:40

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Published on: July 31, 2010

Multiplexed four-channel rectilinear ion trap mass spectrometer.

Sameer Kothari1, Qingyu Song, Yu Xia

  • 1Department of Chemistry, Purdue University, West Lafayette, Indiana 47907-2084, USA.

Analytical Chemistry
|January 29, 2009
PubMed
Summary

A novel four-channel multiplexed mass spectrometer using rectilinear ion trap (RIT) analyzers was developed. This instrument enhances analytical throughput and information content for complex sample analysis.

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Area of Science:

  • Analytical Chemistry
  • Instrumentation Science
  • Mass Spectrometry

Background:

  • Multiplexed mass spectrometry offers potential for increased throughput and information gain.
  • Rectilinear ion traps (RIT) provide a compact and efficient mass analysis technology.

Purpose of the Study:

  • To design, construct, and characterize a novel four-channel multiplexed mass spectrometer system.
  • To enable simultaneous analysis of multiple samples or diverse ionization methods for a single sample.
  • To improve analytical efficiency and data richness in mass spectrometry.

Main Methods:

  • Development of a four-channel system with parallel atmospheric pressure ion (API) sources and RIT mass analyzers.
  • Implementation of shared electronics and a common vacuum system to minimize footprint and complexity.
  • Application of supplementary direct current signals for mass offset correction and a multiplexed automatic gain control (AGC) scheme for independent trap control.

Main Results:

  • Successful characterization of the instrument over the mass-to-charge range of 150 to 1300 Th.
  • Demonstration of independent ion population control and tandem mass spectrometry with a 1 Th isolation window.
  • Validation of dual operational modes: enhanced information content via varied ionization and higher throughput via parallel sample analysis.

Conclusions:

  • The developed four-channel RIT mass spectrometer is a versatile platform for both high-throughput and in-depth sample analysis.
  • The system effectively addresses challenges in mass accuracy and ion population control for multiplexed analysis.
  • The instrument shows promise for applications in fields such as lipidomics and drug analysis.