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Updated: Jun 26, 2026

Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing
Published on: July 5, 2019
[Sequencing on products of Oncomelania hupensis through simple sequence repeat anchored polymerase chain reaction
Jun-tao Guo1, Yi-biao Zhou, Jian-guo Wei
1Department of Epidemiology, School of Public Health, Fudan University, Shanghai 200032, China.
Objective:
To analyze the sequence of microsatellite and the flanking sequence from four populations of Oncomelania hupensis.
Methods:
We cloned 159 SSR-PCR amplification products of a commonly used primer, (CA)(8RY), using O. hupensis genomic DNA as template, and sequenced 82 products.
Results:
The sequences obtained were novel O. hupensis genomic sequences but not repeat simple sequence. It was observed that 36 out of 82 clones contained microsatellites between priming sites. The flanking sequences of certain microsatellite were invariant. Both (GA/CT)(n) and (TTAGGG/CCCTAA)(n) were found in four populations of O. hupensis. However, (CAA)(n) were found only in O. hupensis from Fuqing, Fujian province and (TCTCTG)(n) were found only in O. hupensis from Guichi, Anhui province and (GAA/TTC)(n), (CAA/TTG)(n), (CAT)(n) were found only in O. hupensis from Puge, Sichuan province.
Conclusion:
The results obtained by SSR-PCR should not be interpreted as the amplification of microsatellite loci, and analytical rules similar to those for Random Amplified Polymorphic DNA should be used. SSR-PCR could not make the most of the priority of microsatellite. It seems better to amplify the microsatellites with the primers designed on the basis of the flanking sequence.
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