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Oral Biofilm Formation on Different Materials for Dental Implants
Published on: June 24, 2018
The composition of enamel salivary films is different from the ones formed on dental materials
1Biomedical Laboratory Science and Technology, Faculty of Health and Society, Malmö University, Malmö, Sweden. ida.svendsen@mah.se
Abstract:
This study utilized two-dimensional gel electrophoresis (2DE) to illustrate the compositional differences between in vitro salivary conditioning films (denoted pellicles) formed on human enamel as well as on the dental materials titanium and poly(methyl methacrylate). The salivary pellicles were formed by immersing each surface in individual tubes containing small volumes of freshly collected whole saliva. Saliva remaining in the tubes after the pellicle formation for 2 h was visualized by means of 2DE and silver staining. The results showed that the protein patterns in 2DE of the liquid phase of saliva left after the exposure to the respective surfaces, regarding proteins <100 kDa in size, were different depending on the surface used. Several protein groups and/or individual proteins were shown to be distinct for each surface used.
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