[Studies on the effect of Ginkgo biloba extracts on NF-kappaB pathway]
Guo-Hao Li1, Xiu-Xia Lei, Yan-Mei Yi
1The first Municipal People's Hospital of Guangzhou, Guangzhou 510180, China. ligh999@yahoo.com.cn
Objective:
To investigate NF-kappaB and IkappaBalpha activities in HL-60 induced by TNF-alpha in order to understand the molecular mechanism of GbE in asthma treatment.
Methods:
The amount of IkappaBalpha in HL-60 cells stimulated by TNF-alpha and GbE was measured by western blotting. Plasmid pNF-kappaB-LuC was transfected and NF-kappaB activity was analyzed by measuring the expression level of luciferase.
Results:
It showed in the luciferase assay that the activity of NF-kappaB could significantly be suppressed in HL-60 cells after the pretreatment with CGbE. However, the phosphorylation and subsequent degradation of IKBalpha induced by TNF-alpha can not be inhibited in HL-60 cells even we prolonged the treatment time or increased the concentration of GhE.
Conclusion:
GhE can suppress the NF-kappaB gene expression actively on independent of NIK/ IKK/ IkappaBalpha pathway in HL-60 cells.
