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Related Experiment Video

Updated: Jun 26, 2026

Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection

Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection

Published on: December 15, 2015

A simple and efficient approach to reversed-phase HPLC method screening.

Kallol M Biswas¹, Bryan C Castle, Bernard A Olsen

¹Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285, USA.

Journal of Pharmaceutical and Biomedical Analysis

|February 3, 2009

Summary

This summary is machine-generated.

This study presents an efficient four-column high-performance liquid chromatography (HPLC) method screening strategy for pharmaceutical analysis. The approach enables rapid development of analytical methods for drug quality verification, successfully applied to 40 compounds.

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Automated HPLC Separation Using LC-Mate: An Integrated Repetitive Autosampler and Fraction Collector for Microscale Purification

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Published on: February 27, 2026

Area of Science:

Analytical Chemistry
Pharmaceutical Analysis
Chromatography

Background:

Developing efficient analytical methods is crucial for pharmaceutical quality control.
Traditional HPLC method development can be time-consuming and resource-intensive.
A streamlined screening strategy is needed to accelerate the analysis of drug substances and products.

Purpose of the Study:

To present an efficient HPLC method screening strategy using a limited number of columns.
To establish a rapid early method development approach and a robust commercial method development process.
To demonstrate the practicality and orthogonality of the proposed screening strategy.

Main Methods:

A four-column screening strategy was developed for HPLC method development.
A two-column approach was used for early-stage method development.
Mobile phases included acetonitrile/methanol with low and high pH buffers (trifluoroacetic acid and ammonium hydroxide).
A system suitability check using commercial compounds was implemented for troubleshooting and performance verification.

Main Results:

The strategy successfully developed assay and impurity methods for 40 pharmaceutical compounds.
The chosen columns demonstrated practicality and orthogonality in the screening process.
The system suitability check proved effective for ensuring adequate system performance.

Conclusions:

The presented HPLC method screening strategy is efficient and practical for pharmaceutical analysis.
The four-column approach facilitates the development of robust analytical methods for drug quality control.
This strategy can accelerate the verification of drug substance and drug product quality.