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Related Concept Videos

IR Frequency Region: Fingerprint Region01:03

IR Frequency Region: Fingerprint Region

IR spectra are divided into two main regions: the diagnostic region and the fingerprint region. The diagnostic region of the spectrum lies above 1500 cm−1. The absorptions resulting from single-bond vibrations of the N–H, C–H, and O–H stretch at higher wavenumbers and appear on the left side of the spectrum. The stretching absorptions of the C≡C and C≡N occur between 2100–2300 cm−1. In contrast, those arising from stretching absorptions of the C=O, C=N, and C=C occur between 1600–1850 cm−1.
The...
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

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Related Experiment Video

Updated: Jun 26, 2026

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation (BiFC-PALM)
12:42

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation (BiFC-PALM)

Published on: December 22, 2015

Integrating sequence with FPC fingerprint maps.

William Nelson1, Carol Soderlund

  • 1Arizona Genomics Computational Laboratory, BIO5 Institute, University of Arizona, Tucson, AZ, USA.

Nucleic Acids Research
|February 3, 2009
PubMed
Summary
This summary is machine-generated.

The FPC software package integrates bacterial artificial chromosome (BAC) fingerprint maps, BAC end sequences (BESs), and draft genomic sequences. It offers modules for aligning sequences, determining minimal tiling paths, and integrating draft sequences for enhanced genomic analysis.

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Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
10:14

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Published on: September 2, 2020

Area of Science:

  • Genomics
  • Bioinformatics

Background:

  • Advances in clone fingerprinting and draft sequencing are common.
  • Species increasingly possess bacterial artificial clone (BAC) fingerprint maps, BAC end sequences (BESs), and draft genomic sequences.

Purpose of the Study:

  • To present the FPC (fingerprinted contigs) software package, designed to maximize the utility of genomic resources.
  • To introduce new modules and parallelization features for efficient genomic data analysis.

Main Methods:

  • Utilizes three modules: BSS (blast some sequence), MTP (minimal tiling path), and DSI (draft sequence integration).
  • Employs sequence and fingerprint data for minimal tiling path determination.
  • Aligns draft sequences to FPC contigs, identifying discrepancies.

Main Results:

  • The BSS module facilitates viewing draft sequence alignments to BESs.
  • The MTP module determines minimal tiling paths using sequence and fingerprints.
  • The DSI module integrates draft sequences with FPC contigs, highlighting potential discrepancies.
  • FPC supports high-throughput fingerprint map generation through parallelized functions.

Conclusions:

  • The FPC V9.3 software package effectively integrates and analyzes diverse genomic data.
  • Parallelization of intensive functions enables high-throughput analysis on multi-CPU systems.
  • FPC software is freely available, promoting broader use in genomic research.