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Updated: Jul 28, 2026

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
Determination of ampicillin in biological fluids by coupled-column liquid chromatography and post-column
K Lanbeck-Vallén1, J Carlqvist, T Nordgren
1Department of Bioanalysis, Astra Research Centre AB, Södertälje, Sweden.
Abstract:
A rapid and sensitive high-performance liquid chromatographic method has been developed for the determination of ampicillin in plasma and urine. The method involves a simple deproteinization step and separation on a coupled-column chromatographic system followed by post-column derivatization and fluorescence detection. The method has been used for the determination of ampicillin in various clinical studies. The high sensitivity makes it especially useful for small sample volumes, e.g. samples from pediatric patients.
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