Programmed subcellular release for studying the dynamics of cell detachment
Bridget Wildt1, Denis Wirtz, Peter C Searson
1Department of Materials Science and Engineering, Johns Hopkins University, 3400 N. Charles St., Baltimore, MD 21218, USA.
Nature Methods
|February 3, 2009
Abstract:
Cell detachment is central to a broad range of physiopathological changes, but there are no quantitative methods to study this process. Here we report programmed subcellular release, a method for spatially and temporally controlled cellular detachment, and present quantitative results of the detachment dynamics of 3T3 fibroblasts at the subcellular level.
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