Specific detection of oxytetracycline using DNA aptamer-immobilized interdigitated array electrode chip.

Area of Science:

• Electrochemistry
• Biosensing
• Analytical Chemistry

Background:

• Oxytetracycline (OTC) is an antibiotic requiring sensitive detection methods.
• Existing methods for tetracycline antibiotic detection can lack specificity.
• Development of novel electrochemical sensors is crucial for accurate antibiotic monitoring.

Purpose of the Study:

• To develop a novel electrochemical sensing system for the specific detection of oxytetracycline (OTC).
• To create a biosensor capable of discriminating OTC from structurally similar tetracyclines (TCs).
• To evaluate the performance and dynamic range of the developed sensor.

Main Methods:

• Immobilization of single-stranded DNA (ssDNA) aptamers on a gold interdigitated array (IDA) electrode chip.
• Utilizing cyclic voltammetry (CV) and square wave voltammetry (SWV) to measure electrochemical signals.
• Analyzing current decrease caused by OTC binding, using a ferro- and ferricyanide redox probe.

Main Results:

• The developed electrochemical biosensor demonstrated high specificity for OTC, distinguishing it from doxycycline (DOX) and tetracycline (TET).
• The sensor showed a significant decrease in current upon binding of OTC, DOX, or TET.
• The dynamic range for OTC detection was established as 1-100 nM.

Conclusions:

• The ssDNA aptamer-based electrochemical sensor provides a highly specific and sensitive method for OTC detection.
• The developed biosensor is effective in differentiating OTC from other common tetracyclines.
• This technology holds promise for accurate antibiotic monitoring in various applications.