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Monitoring organelle turnover in yeast using fluorescent protein tags

Rodney J Devenish1, Mark Prescott, Kristina Turcic

  • 1Department of Biochemistry and Molecular Biology, and ARC Centre of Excellence in Structural and Functional Microbial Genomics, Monash University, Clayton Campus, Melbourne, Victoria, Australia.

Methods in Enzymology
|February 3, 2009
PubMed
Abstract

No abstract available in PubMed .

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Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...