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Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds
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Published on: February 14, 2018

In vitro activities of fluconazole and voriconazole against clinical isolates of Candida spp. determined by disk

Narcisa Mandras1, Vivian Tullio, Valeria Allizond

  • 1Department of Public Health and Microbiology, University of Turin, Turin, Italy.

Antimicrobial Agents and Chemotherapy
|February 4, 2009
PubMed

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Amphotericin B is a broad-spectrum antifungal agent that exploits structural differences between fungal and mammalian cell membranes. Its amphipathic structure—featuring a hydrophobic polyene-lactone ring and a hydrophilic region containing mycosamine and carboxylic acid groups—enables selective binding to ergosterol, a sterol predominantly found in fungal plasma membranes. This selective interaction underlies the drug’s antifungal activity, although weak binding to cholesterol contributes to...