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Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...

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Related Experiment Video

Updated: Jun 26, 2026

Multimodal Optical Imaging Platform for Studying Cellular Metabolism
04:47

Multimodal Optical Imaging Platform for Studying Cellular Metabolism

Published on: June 6, 2025

A multimodal platform for nonlinear optical microscopy and microspectroscopy.

Hongtao Chen1, Haifeng Wang, Mikhail N Slipchenko

  • 1Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA.

Optics Express
|February 4, 2009
PubMed
Summary
This summary is machine-generated.

We developed an easy-to-use multimodal nonlinear optical microscopy platform for advanced biological imaging. This system enables Coherent Anti-Stokes Raman Scattering (CARS) and other techniques for detailed tissue and cell analysis.

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Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

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Area of Science:

  • Biomedical Optics
  • Microscopy
  • Molecular Imaging

Background:

  • Multimodal nonlinear optical microscopy offers unique insights into complex biological structures.
  • Existing techniques can be complex to operate and require multiple laser sources.

Purpose of the Study:

  • To present a simplified, single-laser-source approach for multimodal nonlinear optical microscopy.
  • To demonstrate the platform's capability for imaging diverse biological samples.

Main Methods:

  • Utilized a single laser source comprising an 80 MHz femtosecond laser, optical parametric oscillator (OPO), and PPLN crystal.
  • Integrated Coherent Anti-Stokes Raman Scattering (CARS), Two-Photon Fluorescence (TPF), Second Harmonic Generation (SHG), and Third Harmonic Generation (THG) imaging.

Main Results:

  • Successfully performed vibrationally resonant CARS imaging of myelin sheath in spinal tissues.
  • Demonstrated CARS imaging of lipid bodies in live cells.
  • Conducted multimodal nonlinear optical imaging and microspectroscopy of liver tissues.

Conclusions:

  • The developed platform provides an accessible and versatile tool for advanced biological imaging.
  • This approach facilitates detailed analysis of tissue composition and cellular structures.