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Bioreactor Design and Operational System01:29

Bioreactor Design and Operational System

Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
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A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
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Published on: December 25, 2015

The Envirostat - a new bioreactor concept.

Hendrik Kortmann1, Paris Chasanis, Lars M Blank

  • 1ISAS - Institute for Analytical Sciences, Bunsen-Kirchhoff-Str. 11, D-44139, Dortmund, Germany.

Lab on a Chip
|February 5, 2009
PubMed
Summary
This summary is machine-generated.

A novel microfluidic bioreactor, the Envirostat, enables single-cell resolution and constant environmental conditions for cellular physiology studies. This technology overcomes population effects, allowing precise observation of individual cell responses to perturbations.

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Area of Science:

  • Cellular and Molecular Biology
  • Biotechnology
  • Microfluidics

Background:

  • Quantitative cellular physiology is crucial but hindered by population effects in bulk cultures.
  • Microfluidic devices offer potential for precise control over cellular microenvironments.
  • Single-cell resolution is essential to understand individual cell behavior and responses.

Purpose of the Study:

  • To develop and validate a microfluidic bioreactor, the Envirostat, for single-cell resolution and stable culture conditions.
  • To investigate and control environmental parameters, including temperature and medium composition, for single-cell cultivation.
  • To demonstrate the applicability of the Envirostat for long-term single-cell studies.

Main Methods:

  • Construction and operation of the Envirostat microfluidic bioreactor.
  • Contactless cell trapping using negative dielectrophoresis (nDEP).
  • Integration of a Peltier device for precise temperature control, accounting for Joule heating effects.
  • Computational fluid dynamics (CFD) simulations to estimate medium composition changes.
  • Long-term cultivation of Saccharomyces cerevisiae at the single-cell level.

Main Results:

  • The Envirostat successfully maintained constant environmental conditions and provided single-cell resolution.
  • Joule heating effects from nDEP were quantified and integrated into temperature control.
  • CFD simulations confirmed negligible medium composition changes (<0.001%) affecting cell physiology.
  • Single Saccharomyces cerevisiae cells were cultivated for over four generations.

Conclusions:

  • The Envirostat is a viable tool for single-cell research, enabling precise control over cellular microenvironments.
  • This technology facilitates the study of cellular physiology and responses to perturbations at the individual cell level.
  • The Envirostat has the potential to uncover biological phenomena previously masked by population effects.