Fluorescent amino acids: advances in protein-extrinsic fluorophores
Alan Roy Katritzky1, Tamari Narindoshvili
1Center for Heterocyclic Compounds, Department of Chemistry, University of Florida, Gainesville, FL 32611-7200, USA. katritzky@chem.ufl.edu
Abstract:
Fluorescent labeling is today of paramount importance to biological studies and numerous chemical dyes are used extensively to label biological specimens. This perspective highlights interesting aspects of fluorescent labeling by fluorescent peptides and small organic fluorophores that can be incorporated into proteins by genetic fusion to produce a fluorescent label. While many fluorescence applications rely on the use of intrinsic fluorophores, the development of new extrinsic fluorophores remains an essential element for the design of new fluorescent probes.
More Related Videos
Related Concept Videos
Protein Dynamics in Living Cells
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Reporter Genes
Commonly used reporter...
Tagging and Fusion Proteins


