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Ionic-complementary peptide-modified highly ordered pyrolytic graphite electrode for biosensor application.

Hong Yang¹, Shan-Yu Fung, Wei Sun

¹Dept. of Chemical Engineering, University of Waterloo, Waterloo, ON, Canada N2L 3G1.

Biotechnology Progress

|February 6, 2009

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Ionic-complementary peptides, like EFK16-II, create ordered nanofibers on electrodes, enhancing biomaterial applications. This modification enables sensitive glucose biosensing with immobilized glucose oxidase (GOx).

Area of Science:

Bionanotechnology
Biomaterials Science
Electrochemistry

Background:

Ionic-complementary peptides offer novel biomaterial properties for advanced applications.
Modifying electrode surfaces is crucial for developing sensitive biosensors.
Highly ordered pyrolytic graphite (HOPG) is a common electrode material in electrochemical studies.

Purpose of the Study:

To investigate the modification of a HOPG electrode with ionic-complementary peptide EFK16-II.
To characterize the electrochemical properties of the peptide-modified electrode.
To evaluate the performance of an enzyme-based glucose biosensor fabricated on the modified electrode.

Main Methods:

Surface modification of HOPG electrode with EFK16-II peptide.
Characterization of peptide nanofibers using scanning electron microscopy (SEM) (implied).

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Electrochemical analysis using cyclic voltammetry (CV) with ferricyanide/ferrocyanide redox couple.

Immobilization of glucose oxidase (GOx) onto the modified electrode.

Amperometric detection of glucose at a specific applied potential.

Main Results:

EFK16-II formed oriented peptide nanofibers on the HOPG electrode surface.
Surface wettability of the HOPG electrode was significantly improved.
The modified electrode showed efficient electron transfer at slow scan rates but some blockage at high scan rates.
The GOx-immobilized biosensor exhibited linear glucose detection up to 7.5 mM with high sensitivity (11.3 nA/(mM mm(2))).
The biosensor demonstrated good affinity for glucose (K(m) = 6.8 mM) and notable storage/operational stability.

Conclusions:

Ionic-complementary peptides are effective in modifying electrode surfaces for biomolecular sensing.
The EFK16-II nanofiber-modified electrode provides a promising platform for enzyme immobilization.
This approach holds potential for developing advanced biosensors for diagnostics.