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Updated: Jun 25, 2026

Development of a More Sensitive and Specific Chromogenic Agar Medium for the Detection of Vibrio parahaemolyticus and Other Vibrio Species
Published on: November 8, 2016
Detection of marine pathogenic bacterial Vibrio species by multiplex polymerase chain reaction (PCR)
1Department of Biology, Sangmyung University, Seoul-110-743, Korea. mnkim@smu.ac.kr
Abstract:
Polymerase chain reactions (PCR) were performed to accomplish quick and accurate detection of Vibrio species. The primers prepared with 16S-23S rDNA intergenic spacer (IGS) region exhibited an excellent species-specificity for Vibrio sp and detected Vibrio sp more successfully than the conventional culture method. Multiplex PCR was also fruitful not only forthe identification of the 5 standard Vibrio sp simultaneously but also for the detection of Vibrio spin the samples collected from the natural environment.
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