Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

Electron Microscope Tomography and Single-particle Reconstruction

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

Two-Dimensional Microscopy in Microbiology

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

Overview of Microscopy Techniques

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...

Imaging Biological Samples with Optical Microscopy

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Gaussian Process-based prediction of memory performance and biomarker status in ageing and Alzheimer's disease-A systematic model evaluation.

Medical image analysis·2023

Same author

Amphetamine or skin cream? The impact of the sampling site on the concentration of controlled substances: a case report.

International journal of legal medicine·2022

Same author

The Chilean socio-ethno-genomic cline.

Biodemography and social biology·2021

Same author

Time-of-flight mass spectrometry of particle emission during irradiation with slow, highly charged ions.

The Review of scientific instruments·2021

Same author

Inducible expression of human <i>C9ORF72</i> 36x G<sub>4</sub>C<sub>2</sub> hexanucleotide repeats is sufficient to cause RAN translation and rapid muscular atrophy in mice.

Disease models & mechanisms·2021

Same author

Towards a European health research and innovation cloud (HRIC).

Genome medicine·2020

Search research articles

Related Experiment Video

Updated: Jun 25, 2026

Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy

Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy

Published on: November 21, 2016

Simple 3D images from fossil and recent micromaterial using light microscopy.

J T Haug¹, C Haug, A Maas

¹Biosystematic Documentation, University of Ulm, Helmholtzstrasse 20, D-89081 Ulm, Germany. joachim.haug@uni-ulm.de

Journal of Microscopy

|February 7, 2009

Summary

This study introduces a simple 3D imaging technique for small fossils and specimens using a microscope and free software. It enables non-destructive 3D documentation of valuable or limited research material.

More Related Videos

Design and Building of a Customizable, Single-Objective, Light-Sheet Fluorescence Microscope for the Visualization of Cytoskeleton Networks

Design and Building of a Customizable, Single-Objective, Light-Sheet Fluorescence Microscope for the Visualization of Cytoskeleton Networks

Published on: January 26, 2024

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

Published on: May 27, 2008

Related Experiment Videos

Last Updated: Jun 25, 2026

Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy

Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy

Published on: November 21, 2016

Design and Building of a Customizable, Single-Objective, Light-Sheet Fluorescence Microscope for the Visualization of Cytoskeleton Networks

Design and Building of a Customizable, Single-Objective, Light-Sheet Fluorescence Microscope for the Visualization of Cytoskeleton Networks

Published on: January 26, 2024

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

Published on: May 27, 2008

Area of Science:

Paleontology
Microscopy
3D Imaging

Background:

Traditional 3D investigation methods can be destructive or require specialized equipment.
Documenting small-scale fossil and Recent material in 3D is crucial for research.

Purpose of the Study:

To present an accessible and non-destructive technique for extracting 3D information from small specimens.
To provide a cost-effective alternative to existing 3D investigation methods.

Main Methods:

Utilizes a standard light microscope with dark field/differential interference contrast and a digital camera.
Acquires serial images by shifting focus through the specimen.
Processes images using freely available software: CombineZM for image alignment, ImageJ for cropping and edge extraction, and Osirix for 3D reconstruction and export.

Main Results:

Successfully extracts 3D information from small-scale fossil and Recent material.
Generates high-depth-of-field images and exportable 3D formats like Quicktime VR (QTVR) and stereo images.
The process is largely automated and requires minimal specialized hardware.

Conclusions:

This technique offers an easy-to-apply, safe, and non-destructive method for 3D documentation of specimens.
It is particularly valuable for limited, type, or sensitive material where preparatory techniques are not feasible.
The accessibility of required hardware and free software makes this method widely applicable in research labs.