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Related Experiment Video

Updated: Jun 25, 2026

Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord
12:23

Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord

Published on: May 25, 2010

DSCAM functions as a netrin receptor in commissural axon pathfinding.

Guofa Liu1, Weiquan Li, Lei Wang

  • 1Department of Neurology and Robert H. Lurie Comprehensive Cancer Center, Center for Genetic Medicine, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA.

Proceedings of the National Academy of Sciences of the United States of America
|February 7, 2009
PubMed
Summary

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Down syndrome cell adhesion molecule (DSCAM) acts as a receptor for Netrin-1, guiding commissural axons during development. This study reveals DSCAM

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Molecular Biology

Background:

  • Down syndrome cell adhesion molecule (DSCAM) is crucial for neuronal development, including axon guidance and dendrite arborization.
  • The precise function of DSCAM in vertebrate nervous system development remains largely uncharacterized.

Purpose of the Study:

  • To elucidate the role of DSCAM in vertebrate axon guidance.
  • To investigate the interaction between DSCAM and Netrin-1 signaling pathways.

Main Methods:

  • Utilized siRNA-mediated knockdown of DSCAM in vitro and in ovo.
  • Employed overexpression of a mutant DSCAM lacking its intracellular domain.
  • Analyzed Netrin-1-induced axon outgrowth, commissural axon turning, and phosphorylation of Fyn and Pak1.

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Assaying the Ability of Diffusible Signaling Molecules to Reorient Embryonic Spinal Commissural Axons
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Assaying the Ability of Diffusible Signaling Molecules to Reorient Embryonic Spinal Commissural Axons

Published on: March 8, 2010

Retrograde Tracing of Drosophila Embryonic Motor Neurons Using Lipophilic Fluorescent Dyes
08:25

Retrograde Tracing of Drosophila Embryonic Motor Neurons Using Lipophilic Fluorescent Dyes

Published on: January 12, 2020

Related Experiment Videos

Last Updated: Jun 25, 2026

Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord
12:23

Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord

Published on: May 25, 2010

Assaying the Ability of Diffusible Signaling Molecules to Reorient Embryonic Spinal Commissural Axons
09:28

Assaying the Ability of Diffusible Signaling Molecules to Reorient Embryonic Spinal Commissural Axons

Published on: March 8, 2010

Retrograde Tracing of Drosophila Embryonic Motor Neurons Using Lipophilic Fluorescent Dyes
08:25

Retrograde Tracing of Drosophila Embryonic Motor Neurons Using Lipophilic Fluorescent Dyes

Published on: January 12, 2020

Main Results:

  • DSCAM is expressed on commissural axons and interacts with the guidance cue Netrin-1.
  • DSCAM knockdown or signaling blockage inhibits Netrin-1-mediated axon outgrowth and turning.
  • DSCAM alone, independent of DCC, mediates Netrin-1 signaling by activating Fyn and Pak1 phosphorylation.

Conclusions:

  • Vertebrate DSCAM plays an essential role in axon guidance, functioning as a receptor for Netrin-1.
  • This finding highlights a previously unrecognized complexity in the receptors mediating vertebrate Netrin-1 signaling.