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Updated: Jun 25, 2026

An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides
Published on: November 29, 2016
GelBandFitter--a computer program for analysis of closely spaced electrophoretic and immunoblotted bands
Mihail I Mitov1, Marion L Greaser, Kenneth S Campbell
1Department of Physiology, University of Kentucky, Lexington, KY 40536-0298, USA.
Abstract:
GelBandFitter is a computer program that uses non-linear regression techniques to fit mathematical functions to densitometry profiles of protein gels. This allows for improved quantification of gels with partially overlapping and potentially asymmetric protein bands. The program can also be used to analyze immunoblots with closely spaced bands. GelBandFitter was developed in Matlab and the source code and/or a Windows executable file can be downloaded at no cost to academic users from http://www.gelbandfitter.org.
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